I have bought windows hosting from enom for access with asp use, out site working on local computer normally, but when we upload database and site to server its not working, i feel that there is connection problem with database.
I asked this to enom via ticket and asking continous from the last one week, but they dont seems to support, just asked me i am not their direct reseller, and asked to contact nirmani for help, but nirmani dont sell windows hosting, they only buy domain for enom, enom dont try to realize this problem.
I don't know what you mean by 554 but the error that I see now tells me that the path to the database is wrong. Instead of 'E:\userdirs\wwwroot\db\shopdb.mdb' it should be something like 'E:\userdirs\wwwroot\[your site's folder]\db\shopdb.mdb'. I suggest you find out the actual path and hard code it.
You probably do not see the error message because you are using Internet Explorer with friendly errors turned on.
To disable go to Tools/Internet Options.
Click on the Advanced tab.
In the Browsing area look for uncheck "Show friendly HTTP error messages", uncheck "Show friendly URLs" and check "Display a notification about every script error".